Comparative Value Of Real Time And Conventional Pcr

Comparative Value of Real Time and Conventional PCR in Antenatal Gender Determination Naglaa Ibrahim Azab1, *, Rabab Fawzi Salim1, Mohamed A. Hadi Farag2 1 Department of Medical Biochemistry, Faculty of Medicine, Benha University, Benha – Egypt. 2 Department of Gynecology and Obstetrics, Faculty of Medicine, Benha University, Benha – Egypt. *Corresponding author naglaa1270@yahoo.com, naglaa.azab@fmed.bu.edu.eg ABSTRACT Invasive procedures including chorionic villus sampling and amniocentesis in sex-linked diseases increase the risk of fetal loss. Therefore, Noninvasive fetal gender determination using cell-free fetal DNA (cffDNA) in maternal plasma may be promising. Fifty pregnant women with gestational age between sixth to tenth weeks were included. cffDNA were extracted from maternal plasma and amplified by real time PCR and conventional PCR for detection of SRY, DYS14 and DAZ genes as specific genetic markers for male-bearing pregnancies. In general, sensitivity and specificity of real time PCR was better than conventional PCR. However, sensitivity of DYS14 gene and specificity of SRY gene by real time PCR was equal to those of conventional PCR. Sensitivity of DYS14 gene was the highest and sensitivity of SRY gene was the lowest. However, combination of the three Y-chromosome sequences in the diagnosis increased the test accuracy, which is suitable for clinical application. Keywords: Fetal gender determination, cell free fetal DNA, SRY gene, DYS14 gene, DAZ gene,Show MoreRelatedMice Were Fed A Western Type For Therapeutic Ldlr And Vldlr Liver Gene Transfers1429 Words   |  6 Pagesperfused with PBS through the left ventricle, and tissue samples were collected in post-mortem examinations for histological, immunohistological, and DNA/RNA level analyses. Some of the mice receiving the T2/lacZ vectors were sacrificed at the day 3 time point post gene transfers and were used for the characterization of gene transfer efficiency. Mice aortas were carefully dissected from aortic arch to bifurcation and fixed in 4 % PFA-PBS overnight. The fixed aortas were opened longitudinally, attachedRead MoreTherapy Promotes Venous Thromboembolism Through The Induction Of Pai 13287 Words   |  14 Pages05; Figure 1A) in an IVC stenosis model. Next, we analyzed thrombosis in the saphenous vein following injury with ferric chloride and found that mice carrying A549 tumors that were treated with bevacizumab showed significantly shortened occlusion times compared with those of vehicle-treated mice in this venous thrombosis model (410.8 ±47.3 seconds versus 684.4 ±65.2 seconds in the vehicle group, p0.05; Figure 1B). In fact, clinical-phase studies have shown that treatment with a VEGF receptor inhibitorRead MoreBCH190 Essay14810 Words   |  60 Pages(C) the theory of evolution is easily disproven (D) complex structures can be developed (E) phylogeny acquires teleology 8. To exist, life requires organization and organization requires energy, which means that life’s molecules breakdown over time. Literally you are not the ‘same’ person you were seven years ago, or even seven days ago. Life maintains itself by the process of:    (A) turnover (B) spontaneous combustion (C) internal combustion (D) hopeful purpose (E) totally randomRead MoreQuality Improvement328284 Words   |  1314 PagesEditor: Sandra Dumas Senior Designer: Kevin Murphy New Media Editor: Lauren Sapira Editorial Assistant: Mark Owens Production Management Services: Elm Street Publishing Services Composition Services: Aptara, Inc. This book was typeset in 10/12 Times by Aptara, Inc., and printed and bound by R. R. Donnelley (Jefferson City). The cover was printed by R. R. Donnelley (Jefferson City). The paper in this book was manufactured by a mill whose forest management programs include sustained yield harvestingRead MoreAnz Bank142091 Words   |  569 Pagesfunctions are helping ANZ build economies of scale, increase our speed to market and strengthen the operating risk control environment for the business. The  Group’s regional delivery centres provide full service regional coverage across our operating time zones helping to drive lower unit costs, improve quality and lower  risk. Our business risk profile improved, with a continuing shift to investment-grade clients and shorter tenor Trade Finance, and greater earnings diversification across products